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- Path: sparky!uunet!spool.mu.edu!agate!biosci!CLVAX1.CL.MSU.EDU!preissj
- From: preissj@CLVAX1.CL.MSU.EDU ("J Preiss--Seq Anal")
- Newsgroups: bionet.software
- Subject: Small scale Auto Sequence entry system
- Message-ID: <9301060448.AA14938@net.bio.net>
- Date: 6 Jan 93 04:39:00 GMT
- Sender: daemon@net.bio.net
- Distribution: bionet
- Lines: 61
-
- sequence.pgm
-
- I am looking for a system for automating entry of sequence
- data into the computer in our lab.
-
- We are a midsized (about 12 of us) biochemistry lab who
- sequence 2-10 kb per year to verify clones and site directed
- mutants. We use Sanger di-deoxy sequencing with Sequenase and
- 35-S-dATP on acrylamide gels and X-ray film. We use an IBM clone
- (386-25) and GCG on a VAX for sequence analysis.
-
- Currently we read sequence films on a light box, record data
- on scratch paper, and type it into the computer for analysis.
- This is tedious and error prone.
-
- The lab PI recently asked me to recommend a system to enter
- sequence data into the computer automatically. He wants to spend
- under $1,000- and wants to stick to the traditional autorad
- technology that he feels comfortable with.
-
- I have a vision of a system in mind, but have no idea if
- anything like it exists. I envision a small scanner device to
- enter data into the computer and software which would allow you
- to see the autorad on screen. I would like to see a visual
- picture of the computers choices for reading the sequence which
- could be edited on screen by the user. Data should be in a
- format which could be easily saved as an ASCII file of sequence
- or directly shipped down to the vax for GCG to analyze.
-
- Ideally, I would like some versatility in the system. If I
- had a scanner on hand, I would like to be able to use it to enter
- sequence from old publications or faxed data directly from text
- on paper. I would like the option to digitize pictures of
- agarose gels into the computer to use graphics programs to add
- pointers and text to the gel picture for publication quality
- figures.
-
- This system would require some hardware and some software,
- of which we have neither. The last system I used was purchased
- around 1986 and I no longer have access to it or any of it's
- components. Any suggestions for hardware and/or software for a
- system like this would be greatly appreciated. In leu of my
- dream system, I would also appreciate any recommendations from
- people using small scale automated sequence data entry systems.
- Any warnings or advice will be greatly appreciated.
-
- A summary will be posted back to the net if I get anything
- interesting and helpful back. If I get nothing I will try
- posting to autoseqs, but I don't subscribe to that group.
-
-
- Thanks
-
- Dr. Leonard N. Bloksberg
- PreissJ@clvax1.cl.msu.edu
- Dept. of Biochemistry
- Michigan State University
-
- "Life is weird"
-
-
-