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Asymetrix ToolBook File | 1994-06-16 | 44KB | 299 lines

1000,1000 leavePage leavePage buttonUp buttonUp buttonUp buttonUp buttonUp buttonUp 1000 6000 h,1000 buttonUp buttonUp Elect buttonUp buttonUp nthe 1180, 985 1075, 1630 970, 2395 leavePage leavePage nthe 1180, 985 1075, 1630 970, 2395 syslockScreen d1, 985 d2, 1630 d3, 2395 buttonUp buttonUp Elect buttonUp buttonUp B $ r B $ r buttonUp buttonUp buttonUp buttonUp previous buttonUp buttonUp Times New Roman Times New Roman Times New Roman Times New Roman Times New Roman Arial System -- Puts the mode hides menus file EnterBook Reader sysRuntime c"Edit" c"Help" c"Text" c"File" sysScreenLock LeaveBook EnterBook LeaveBook EnterBook sizetopage sysScreenLock LeaveBook buttonUp buttonUp buttonUp buttonUp previous buttonUp buttonUp "dna1" nthe 185, 3165 leavePage leavePage Hybridisation of DNA strands dna1 155, 3105 155, 2805 155, 2505 155, 2205 buttonUp buttonUp Denature "dna1" nthe 2360, 3195 buttonUp buttonUp Short 1 dna1 155, 2505 155, 2805 155, 3105 155, 3205 buttonUp buttonUp Aligned "dna1" nthe 1715, 3225 buttonUp buttonUp Short 2 -ATGA- -TACT- + graph leavePage leavePage graph Separation Methods for DNA niques Techniques hniques Electrophoresis in polyacrylamide Electrophoresis in agarose Gels - continuous 3D mesh All DNAs have constant charge:mass ratio Try to move at same velocity Gel determines mobility Inversely proportional to M thods for DNA Labelling methods for DNA Specific fragmentation methods for DNA Hybridization techniques Blotting methods graph buttonUp buttonUp graph Graph on graph Log Mr Mobility graph buttonUp buttonUp graph Graph off Button buttonUp buttonUp Elect Techniques Required Acid Techniques Separation methods for DNA Labelling methods for DNA Specific fragmentation methods for DNA Hybridization techniques Blotting methods h DNA fragment of many? nt of many? sysLockscreen radio -440, 1110 8115,960 Line rl leavePage leavePage radio Labelling Methods for DNA Techniques hniques End labelling 3'-end Incubate with Klenow fragment (DNA pol) hods for DNA Labelling methods for DNA Specific fragmentation methods for DNA Hybridization techniques Blotting methods ethods for DNA Labelling methods for DNA Specific fragmentation methods for DNA Hybridization techniques Blotting methods New DNA ".U_? nthe -420, 1110 8115, 960 0, 1600 7500, 1600 750, 2400 6800, 2400 1500, 3015 6180, 3015 Line rl buttonUp buttonUp Binding radio Radioactive DNAddddd Klenow enzyme sysLockscreen 2840,2985 2955,2985 leavePage leavePage Fragmentation Methods for DNA hniques hniques Restriction endonucleases Cleave at defined inverted repeat sequences on techniques Blotting methods pecific fragmentation methods for DNA Hybridization techniques Blotting methods ines mobility inversely proportional to M eparation methods for DNA Labelling methods for DNA Specific fragmentation methods for DNA Hybridization techniques Blotting methods sysLockscreen nthe 3055, 2985 2780, 2985 3155, 2985 2680, 2985 3255, 2985 2580, 2985 3355, 2985 2480, 2985 3455, 2985 2380, 2985 3555, 2985 2280, 2985 3655, 2985 2180, 2985 3755, 2985 2080, 2985 buttonUp buttonUp Animate 5'-GAATTC-3' 3'-CTTAAG-5' AATTC-3' G-5'' 3'-CTTAA'''''''''''' buttonUp buttonUp Return to Start sysLockscreen nthe 1400, 3015 6280, 3015 1300, 3015 6380, 3015 1200, 3015 6480, 3015 1100, 3015 6580, 3015 1000, 3015 6680, 3015 radio 900, 3015 6780, 3015 800, 3015 6880, 3015 700, 3015 6980, 3015 600, 3015 7080, 3015 500, 3015 7180, 3015 buttonUp buttonUp radio NUCLEIC ACID TECHNIQUES "Really quit?"\ f"Yes" SysSuspendMessages buttonUp buttonUp Really quit? Nucleic Acid Techniques Detection of Specific DNA sequences In which organism of many? In which DNA fragment of many?