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1994-08-27
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Document 0762
DOCN M9480762
TI A sensitive promoter assay based on the transcriptional activator Tat of
the HIV-1 virus.
DT 9410
AU Koken SE; van Wamel J; Berkhout B; Department of Virology, University of
Amsterdam, The Netherlands.
SO Gene. 1994 Jul 8;144(2):243-7. Unique Identifier : AIDSLINE MED/94314223
AB We developed a sensitive vector system for the analysis of weak promoter
activities. This promoter assay is based on the transcriptional
activator protein, Tat, of human immunodeficiency virus type 1 (HIV-1).
High-level expression of HIV requires activation in trans by Tat of the
promoter in the long terminal repeat (LTR). Here we describe the
construction of a promoterless pTat vector. Foreign promoter elements
can be inserted upstream from the tat gene, and expression of Tat
protein is measured in trans on a co-transfected LTR-CAT reporter
plasmid. We show that this binary system is more sensitive than standard
pCAT reporter assays.
DE Animal Cell Line Chloramphenicol Acetyltransferase/GENETICS Gene
Products, tat/*GENETICS Genetic Vectors Hela Cells Human HIV Long
Terminal Repeat HIV-1/*GENETICS/METABOLISM Mice Plasmids *Promoter
Regions (Genetics) Rats Sensitivity and Specificity Support, Non-U.S.
Gov't 3T3 Cells JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).