Document 0762
 DOCN  M9480762
 TI    A sensitive promoter assay based on the transcriptional activator Tat of
       the HIV-1 virus.
 DT    9410
 AU    Koken SE; van Wamel J; Berkhout B; Department of Virology, University of
       Amsterdam, The Netherlands.
 SO    Gene. 1994 Jul 8;144(2):243-7. Unique Identifier : AIDSLINE MED/94314223
 AB    We developed a sensitive vector system for the analysis of weak promoter
       activities. This promoter assay is based on the transcriptional
       activator protein, Tat, of human immunodeficiency virus type 1 (HIV-1).
       High-level expression of HIV requires activation in trans by Tat of the
       promoter in the long terminal repeat (LTR). Here we describe the
       construction of a promoterless pTat vector. Foreign promoter elements
       can be inserted upstream from the tat gene, and expression of Tat
       protein is measured in trans on a co-transfected LTR-CAT reporter
       plasmid. We show that this binary system is more sensitive than standard
       pCAT reporter assays.
 DE    Animal  Cell Line  Chloramphenicol Acetyltransferase/GENETICS  Gene
       Products, tat/*GENETICS  Genetic Vectors  Hela Cells  Human  HIV Long
       Terminal Repeat  HIV-1/*GENETICS/METABOLISM  Mice  Plasmids  *Promoter
       Regions (Genetics)  Rats  Sensitivity and Specificity  Support, Non-U.S.
       Gov't  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).