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1994-08-27
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Document 0701
DOCN M9480701
TI CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency
virus-suppressing lymphokine.
DT 9410
AU Ennen J; Findeklee H; Dittmar MT; Norley S; Ernst M; Kurth R;
Paul-Ehrlich Institute, Langen, Germany.
SO Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7207-11. Unique Identifier
: AIDSLINE MED/94316666
AB Following natural and experimental infection by simian immunodeficiency
virus SIVagm of African green monkeys (AGMs), the natural host, there is
no evidence for the development of an immunodeficiency. Within the
framework of our studies on human immunodeficiency virus (HIV)/SIV
pathogenesis, we investigated the influence of CD8 T lymphocytes on
SIVagm replication in AGM CD4 T lymphocytes in vitro. The following
observations were made: (i) Peripheral blood mononuclear cells from both
seronegative and seropositive AGMs contained only a low proportion
(i.e., 10%) of CD4+ lymphocytes, whereas a high proportion (80%) of CD8+
cells was detected. Even after persistent SIVagm infection, CD4 T
lymphocytes do not decrease in number. (ii) The target of in vitro
infection of peripheral blood cells is the CD4+ mononuclear cell (T
lymphocytes, monocytes) and SIVagm infects by binding to the CD4
molecule. (iii) In both naturally and experimentally SIVagm-infected
AGMs the CD4+ T cells and monocytes, but not the CD8+ T cells, harbor
DNA provirus. (iv) Virus reisolation and virus replication of SIVagm in
CD4 T lymphocytes from seropositive AGMs is suppressed in the presence
of autologous CD8 T lymphocytes or a soluble factor produced by these
cells. Taken together, one possible reason for the apathogenicity of the
SIVagm infection in AGMs may be the suppression of virus replication by
a soluble, yet unidentified factor secreted by CD8 lymphocytes
quantitatively dominating among peripheral blood cell populations. We
have tentatively termed this factor immunodeficiency virus-suppressing
lymphokine. In addition, we show that immunodeficiency virus-suppressing
lymphokine from AGMs is able to suppress HIV-1 replication in human CD4+
T cells.
DE Animal Antigens, CD8 Base Sequence Cercopithecus aethiops DNA,
Viral/ANALYSIS Lymphokines/IMMUNOLOGY/*SECRETION Molecular Sequence
Data Monocytes/CYTOLOGY/MICROBIOLOGY Proviruses/GENETICS Simian
Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Support, Non-U.S. Gov't
SIV/*IMMUNOLOGY/PHYSIOLOGY T-Lymphocyte
Subsets/IMMUNOLOGY/MICROBIOLOGY/*SECRETION T4 Lymphocytes/MICROBIOLOGY
Virus Replication JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).