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M94A0032.TXT
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1994-10-01
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Document 0032
DOCN M94A0032
TI [The evaluation of the effect of formol and sodium hypochlorite on the
demonstration of Cryptosporidium oocysts in feces by Heine's method]
DT 9412
AU Modolo JR; Amato Neto V; Braz LM; Lopes CA; Gottschalk AF; Faculdade de
Medicina Veterinaria e Zootecnia de Botucatu,; Universidade Estadual
Paulista, SP, Brazil.
SO Rev Soc Bras Med Trop. 1994 Apr-Jun;27(2):75-7. Unique Identifier :
AIDSLINE MED/94353093
AB Cryptosporidium oocysts were searched by Heine's method in stools of
nine calves with cryptosporidiosis after stool treatment with two
disinfectants, 10% paraformaldehyde solution and 14.5% sodium
hypochlorite solution. After 30 minutes exposition to sodium
hypochlorite solution oocysts became non refractile and acquired a
reddish tinge, making their identification difficult. No morphological
alterations occurred in oocysts after paraformaldehyde treatment. We
recommend paraformaldehyde at 10% concentration as means of human
immunodeficiency virus (HIV) inactivation for routine use in stool
examinations and therefore making safer those type of procedures for
laboratory personnel, when using Heine's method.
DE Animal AIDS-Related Opportunistic Infections/PARASITOLOGY Cattle
Cattle Diseases/PARASITOLOGY Comparative Study
Cryptosporidiosis/PARASITOLOGY Cryptosporidium/*DRUG EFFECTS/ISOLATION
& PURIF English Abstract Evaluation Studies Feces/*PARASITOLOGY
Formaldehyde/*PHARMACOLOGY Human HIV-1 Indicators and Reagents
Methods Sodium Hypochlorite/*PHARMACOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).