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M9480818.TXT
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1994-09-05
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Document 0818
DOCN M9480818
TI Comparison of direct fluorescence antibody and calcofluor white stain
for detection of Pneumocystis carinii in respiratory specimens.
DT 9410
AU Aslanzadeh J; Kowalski I; Stelmach PS; Ryan RW; University of
Connecticut School of Medicine, Farmington.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:625 (abstract no. V-47). Unique
Identifier : AIDSLINE ASM94/94313127
AB Direct fluorescence monoclonal antibody stain (DFA) was compared,
prospectively, with calcofluor white stain (CFW) for diagnosis of
Pneumocystis carinii in 163 respiratory specimens from 97 patients. The
patient population included HIV infection (58%), bone marrow transplant
recipient (10%), immunosuppressed due to chemotherapy (21%) and others
(11%). Nineteen specimens, 12 sputa, 6 BALs, 1 induced sputum, were
positive by DFA. In contrast, only 6 sputa, 5 BALs were positive if one
CFW stained slide was examined per specimen. However, an additional BAL
was positive if two slides were examined per specimen. All specimen
positive by CFW were also positive by DFA. Of 86 Sputa that were
negative by either methods 3 specimens were followed by induced sputum
18 by BAL 6 by lung biopsy and 2 by pleural fluid. All the subsequent
induced sputa, pleural fluids, and lung biopsies were negative by both
methods. However, 4 of 18 subsequent BALs (22%) were positive by both
methods provided at least two CFW stained slides were examined per
specimen. We conclude that, except for expectorated sputum, CFW is a
rapid and inexpensive test to detect P. carinii from most respiratory
specimens.
DE AIDS-Related Opportunistic Infections/*DIAGNOSIS *Benzenesulfonates
Biopsy Bone Marrow Transplantation Bronchoalveolar Lavage
Fluid/*MICROBIOLOGY Comparative Study *Fluorescent Antibody Technique
Human HIV Infections/COMPLICATIONS Immunosuppression/ADVERSE EFFECTS
Lung/*MICROBIOLOGY/PATHOLOGY Pleural Effusion/MICROBIOLOGY
Pneumocystis carinii Infections/*DIAGNOSIS/ETIOLOGY Postoperative
Complications/DIAGNOSIS/MICROBIOLOGY Prospective Studies
Sputum/*MICROBIOLOGY Stains and Staining/METHODS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).