Celestin Apprentice 4

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Text File | 1995-12-17 | 29.0 KB | 524 lines | [TEXT/R*ch]

(Linear) MAP of: testre.gcg check: 2116 from: 1 to: 1501 With 209 enzymes: * MaxCuts: 4 July 1, 1995 12:42 .. S E B a c H Ep A u Do i cu FH M N l 3 pR n o1 oh s s w A nI 4 N0 ka e p I I II I II II I I / ATATAACATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGCGCGTGCTTAACACATGCA 1 ---------+---------+---------+---------+---------+---------+ 60 TATATTGTACTCTCAAACTAGGACCGAGTCCTACTTGCGACGCGCACGAATTGTGTACGT a I * - b Y N M R V * M N A A R A * M Q - c I T * - 1 ---------+---------+---------+---------+---------+---------+ 60 d - e - f Y L M * C M C - S a A u D E l 3 p c w A n i I I I I AGTCGAACGAAGCATTTACTTACGATCCCTTCGGGGTGACGAGTTTATGACTGAGTGGCG 61 ---------+---------+---------+---------+---------+---------+ 120 TCAGCTTGCTTCGTAAATGAATGCTAGGGAAGCCCCACTGCTCAAATACTGACTCACCGC a - b V E R S I Y L R S L R G D E F M T E W R - c - 61 ---------+---------+---------+---------+---------+---------+ 120 d - e - f T S R L M - A B B f s sE B lM t M pc s C Il E m 2o m j Iu I e 4N F e II I I II I I / / GACGGGTGAGTAACGCGTGGGTAACCTACCTTATACAGGGGGACAACAGTTGGAAACGAC 121 ---------+---------+---------+---------+---------+---------+ 180 CTGCCCACTCATTGCGCACCCATTGGATGGAATATGTCCCCCTGTTGTCAACCTTTGCTG a - b T G E * - c - 121 ---------+---------+---------+---------+---------+---------+ 180 d - e - f - B A C s f j p H l e 2 h I P 4 a I I I I I / TGCTAATACCGCATAAGCGCACGATGTTGCATGACAACGTGTGAAAAGATTTATCGGTAT 181 ---------+---------+---------+---------+---------+---------+ 240 ACGATTATGGCGTATTCGCGTGCTACAACGTACTGTTGCACACTTTTCTAAATAGCCATA a M T T C E K I Y R Y - b - c M L H D N V * - 181 ---------+---------+---------+---------+---------+---------+ 240 d - e * Y R M L A R H Q M - f - S S Aa P Ba H Bvu s F S cu D g ca9 h a t e3 p a cI6 A u y fA n I III I I I II I / AAGATGGACCCGCGTCTGATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATC 241 ---------+---------+---------+---------+---------+---------+ 300 TTCTACCTGGGCGCAGACTAATCGATCAACCACTCCATTGCCGAGTGGTTCCGCTGCTAG a K M D P R L I S * - b - c - 241 ---------+---------+---------+---------+---------+---------+ 300 d - e * N T L Y R S V L A V I - f - H B H S H g s B Ga B aBa i t s Ede s use E E r aiI m 9mI I I F eII A 6FI I I I III I III / / AGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTA 301 ---------+---------+---------+---------+---------+---------+ 360 TCATCGGCTGGACTCTCCCACTGGCCGGTGTAACCCTGACTCTGTGCCGGGTTTGAGGAT a - b - c - 301 ---------+---------+---------+---------+---------+---------+ 360 d - e L L R G S L T V P W M - f - BE T S B cc s S f H s T eo p s a h a a fN R p N a H u II I I I I I I / CGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCG 361 ---------+---------+---------+---------+---------+---------+ 420 GCCCTCCGTCGTCACCCCTTATAACGTGTTACCCGCGTTCGGACTACGTCGCTGCGGCGC a - b - c M G A S L M Q R R R V - 361 ---------+---------+---------+---------+---------+---------+ 420 d - e - f - M M H X b BbR K g m o aos p a n I nIa n I I I III I / TGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGATAATGACGGTACC 421 ---------+---------+---------+---------+---------+---------+ 480 ACTCGCTTCTTCATAAAGCCATACATTTCGAGATAGTCGTCCCTTCTATTACTGCCATGG a M * - b M T V P - c S E E V F R Y V K L Y Q Q G R * - 421 ---------+---------+---------+---------+---------+---------+ 480 d - e - f - M B B sS BS s s DTpa sn r a saAc aa F A au1I AB I I IIII II / / TGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGC 481 ---------+---------+---------+---------+---------+---------+ 540 ACTGATTCTTCGTGGCCGATTTATGCACGGTCGTCGGCGCCATTATGCATACCACGTTCG a - b D * M V Q A - c - 481 ---------+---------+---------+---------+---------+---------+ 540 d - e - f - BBT ssa B B apq f s WEI i r III I I // GTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTTTTACAAGTCTGATGTGAAA 541 ---------+---------+---------+---------+---------+---------+ 600 CAATAGGCCTAAATGACCCACATTTCCCTCGCATCCGCCAAAATGTTCAGACTACACTTT a - b L S G F T G C K G S V G G F T S L M * - c - 541 ---------+---------+---------+---------+---------+---------+ 600 d - e - f - C D Dj M S s se m f a aP e c I II I I GCCCGTGGCTCAACCACGGTATTGCATTGGAAACTGTAGAACTAGAGTGTCGGAGGGGTA 601 ---------+---------+---------+---------+---------+---------+ 660 CGGGCACCGAGTTGGTGCCATAACGTAACCTTTGACATCTTGATCTCACAGCCTCCCCAT a - b - c - 601 ---------+---------+---------+---------+---------+---------+ 660 d - e - f * G R Y Q M - T s Ep T Ac5 B s po0 s p oR9 r R III I I // AGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAA 661 ---------+---------+---------+---------+---------+---------+ 720 TCACCTTAAGGATCACATCGCCACTTTACGCATCTATAATCCTCCTTGTGGTCACCGCTT a - b - c M R R Y * - 661 ---------+---------+---------+---------+---------+---------+ 720 d - e - f - B C B p jT s B u H ea p s 1 g Pu G r 0 a II I I I I / GGCGGCTTACTGGACGATTACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATT 721 ---------+---------+---------+---------+---------+---------+ 780 CCGCCGAATGACCTGCTAATGACTGCGACTCCGAGCTTTCGCACCCCTCGTTTGTCCTAA a - b - c - 721 ---------+---------+---------+---------+---------+---------+ 780 d - e * Q R Q P E F A H P A F L I - f - T t Eh B B c1 C s H cBo1 Cj Bp i eaR1 je s2 n feII eP b4 f IIII II II I // / / AGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGCTTCCATAGGGAG 781 ---------+---------+---------+---------+---------+---------+ 840 TCTATGGGACCATCAGGTGCGGCATTTGCTACTTATGATCCACAACCGAAGGTATCCCTC a - b - c M N T R C W L P * - 781 ---------+---------+---------+---------+---------+---------+ 840 d - e L Y G P L G R R L R H I S P T P K W L S - f * T N A E M - B E s c B PT p o R a la 2 R s n eu 4 I a I II I I I TCGGTGCCGCAGCTAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAA 841 ---------+---------+---------+---------+---------+---------+ 900 AGCCACGGCGTCGATTGCGTTATTCATAAGGTGGACCCCTCATGCAAGCGTTCTTACTTT a M K - b - c - 841 ---------+---------+---------+---------+---------+---------+ 900 d - e D T G C S V C Y T N W R P L V N A L I F - f * R L L Y E V Q P T R E C S H F - E T c T s oP S s p AOsSa B p 5 v1pau F s NC M5 N 0 a05n9 a m sj s0 s 9 I9ID6 u F pe e9 p I IIIII I I II II V //// CTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAA 901 ---------+---------+---------+---------+---------+---------+ 960 GAGTTTCCTTAACTGCCCCTGGGCGTGTTCGCCACCTCGTACACCAAATTAAGCTTCGTT a L K G I D G D P H K R W S M W F N S K Q - b - c - 901 ---------+---------+---------+---------+---------+---------+ 960 d * N S A - e S L P I S P S G C L R H L M - f E F S N V P V R V L P P A H P K I R L L - M M F b b o o o k I I I I I CGCGAAGAACCTTACCAAATCTTGACATCCTCTTGACAAACTATGTAATGTAGTCTTTCT 961 ---------+---------+---------+---------+---------+---------+ 1020 GCGCTTCTTGGAATGGTTTAGAACTGTAGGAGAACTGTTTGATACATTACATCAGAAAGA a R E E P Y Q I L T S S * M * - b - c M * - 961 ---------+---------+---------+---------+---------+---------+ 1020 d V R L V K G F R S M - e - f A F F R V L D Q C G R S L S H L T T K R - A B cB B s B es c s c Im g S g IF I I I II TCGGAACAAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG 1021 ---------+---------+---------+---------+---------+---------+ 1080 AGCCTTGTTCTCACTGTCCACCACGTACCAACAGCAGTCGAGCACAGCACTCTACAACCC a - b M V V V S S C R E M L G - c - 1021 ---------+---------+---------+---------+---------+---------+ 1080 d - e - f R F L L S L H H M - B s p 1 B M F H B 2 s s a h m 8 m e u a g 6 A I I I I I I TTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTAGCCAGCATTTTAGGTGGGCACTC 1081 ---------+---------+---------+---------+---------+---------+ 1140 AATTCAGGGCGTTGCTCGCGTTGGGAACAGGAATCATCGGTCGTAAAATCCACCCGTGAG a - b * - c - 1081 ---------+---------+---------+---------+---------+---------+ 1140 d - e - f * T G C R A C G K D K T A L M - B B AH ANNS s s ai F vccm a a tn o aiia W H I4 k IIII I I II I / / TAGGGAGACTGCCCGGGTGAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCC 1141 ---------+---------+---------+---------+---------+---------+ 1200 ATCCCTCTGACGGGCCCACTTGGCCTCCTTCCACCCCTACTGCAGTTTAGTAGTACGGGG a - b M T S N H H A P - c M P L - 1141 ---------+---------+---------+---------+---------+---------+ 1200 d * I M M - e - f - A B f B T s l sP a t I am q E I Al I I I II I I / TTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGTGGGTGAC 1201 ---------+---------+---------+---------+---------+---------+ 1260 AATACTAAACCCGATGTGTGCACGATGTTACCGCATTTGTTTCCCTTCGTTCACCCACTG a - b Y D L G Y T R A T M A * - c M I W A T H V L Q W R K Q R E A S G * - 1201 ---------+---------+---------+---------+---------+---------+ 1260 d - e - f * V R A V I A Y V F P L L H T V - H BB Bi ss pn mm l4 AB II II / / CATGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACAT 1261 ---------+---------+---------+---------+---------+---------+ 1320 GTACTCGTTTAGAGTTTTTATTGCAGAGTCAAGCCTAACATCAGACGTTGAGCTGATGTA a - b M S K S Q K * M - c - 1261 ---------+---------+---------+---------+---------+---------+ 1320 d - e * N P N Y D A V R S C - f M * M - H H iT N iT X ANNS nf r nf m vccm fi u fi n aiia II I II I IIII / / / GAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCT 1321 ---------+---------+---------+---------+---------+---------+ 1380 CTTCGACCTTAGCGATCATTAGCGCTTAGTCTTACAGCGCCACTTATGCAAGGGCCCAGA a - b K L E S L V I A N Q N V A V N T F P G L - c M S R * - 1321 ---------+---------+---------+---------+---------+---------+ 1380 d * Y D R I L I D R H I R E R T - e S A P I A L L R S D S H R P S Y T G P D - f - B U s Rb DNS B r sa sct s G aC aoy m I II III I / // TGTACACACCGCCCGTCACACCATGGGAGTTGGGAATGCCCGAAGTCTGTGACCCAACCG 1381 ---------+---------+---------+---------+---------+---------+ 1440 ACATGTGTGGCGGGCAGTGTGGTACCCTCAACCCTTACGGGCTTCAGACACTGGGTTGGC a - b V H T A R H T M G V G N A R S L * - c M P E V C D P T V - 1381 ---------+---------+---------+---------+---------+---------+ 1440 d K Y V G G T V G H S N P I G S T Q S G V - e Q V C R G D C W P L Q S H G F D T V W G - f * V M P T P F A R L R H G L R - B B f s i r I I TAAGGAGGGAGCAGCCGAAGG....CTCGATAACTGGGGTGAAGTCGTAACAAGGTATCC 1441 ---------+---------+---------+---------+---------+---------+ 1500 ATTCCTCCCTCGTCGGCTTCC....GAGCTATTGACCCCACTTCAGCATTGTTCCATAGG a - b - c R R E Q P K ? ? S I T G V K S * - 1441 ---------+---------+---------+---------+---------+---------+ 1500 d T L L S C G F ? . E I V P T F D Y C P I - e Y P P L L R L ? ? R Y S P H L R L L T D - f L S P A A S P . ? S L Q P S T T V L Y G - T 1501 - 1501 A a - b - c - 1501 - 1501 d R - e - f - Enzymes that do cut: AatII AceIII AciI AflIII AluI AlwI ApoI AvaI AvaII BaeI BanI BbvI BccI BcefI BcgI BfaI BfiI BmgI BplI Bpu10I BsaAI BsaHI BsaJI BsaWI BsbI BscGI BslI BsmI BsmAI BsmBI BsmFI BsoFI Bsp24I Bsp1286I BspEI BspGI BsrI BsrFI BsrGI BssSI BstEII Cac8I CjeI CjeI CjePI CjePI CviJI CviRI DdeI DpnI DsaI EaeI EciI EcoNI EcoO109I EcoRI EcoRII FauI FokI GdiII HaeIII HgaI HgiEII HhaI Hin4I HinfI HphI KpnI MaeII MaeIII MboII MluI MmeI MnlI MseI MspI MspA1I MwoI NciI NcoI NlaIII NlaIV NruI NspI NspV PleI PmlI PshAI Psp5II RsaI SacII SanDI Sau96I Sau3AI ScrFI SfaNI SfcI SmaI SnaBI SspI StyI TaqI TaqII TauI TfiI ThaI TseI Tsp45I Tsp509I TspRI Tth111II UbaCI XmnI Enzymes that do not cut: AccI AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BamHI BanII BbsI Bce83I BclI BglI BglII BpmI Bpu1102I BsaI BsaBI BsaXI BseRI BsgI BsiEI BsiHKAI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstXI BstYI Bsu36I ClaI DraI DraIII DrdI DrdII EagI EarI Eco47III Eco57I EcoRV FseI FspI HaeI HaeII HincII HindIII HpaI MscI MslI MunI NarI NdeI NgoAIV NheI NotI NsiI PacI Pfl1108I PflMI PinAI PmeI Psp1406I PstI PvuI PvuII RcaI RleAI RsrII SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI SpeI SphI SrfI Sse8387I Sse8647I StuI SunI SwaI Tth111I VspI XbaI XcmI XhoI Enzymes excluded; MinCuts: 1 MaxCuts: 4 AciI AluI BbvI BfaI BsaJI BscGI BslI BsoFI Cac8I CjeI CjePI CviJI CviRI DdeI HphI MaeII MaeIII MnlI MspI MwoI NlaIII NlaIV ScrFI TaqI ThaI TseI Tsp45I