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- ***********************************
- * Gamma-thionins family signature *
- ***********************************
-
- The following small plant proteins are evolutionary related:
-
- - Gamma-thionins from the endosperm of wheat (gamma-purothionins) and barley
- (gamma-hordothionins) which are toxic to animal cells and inhibit protein
- synthesis in cell free systems [1].
- - A flower-specific thionin (FST) from tobacco [2].
- - Antifungal proteins (AFP) from the seeds of Brassicaceae species such as
- radish, mustard, turnip and Arabidopsis thaliana [3].
- - Inhibitors of insect alpha-amylases from sorghum [4].
- - Probable protease inhibitor P322 from potato.
- - A germination-related protein from cowpea [5].
- - Anther-specific protein SF18 from sunflower [6]. SF18 is a protein that
- contains a gamma-thionin domain in its N-terminal and a proline-rich C-
- terminal domain.
-
- In their mature form these proteins generally consist of about 45 to 50 amino
- acid residues. As shown in the following schematic representation, these
- peptides contains eight conserved cysteines that are involved in disulfide
- bonds.
-
- +-------------------------------------------+
- | +-------------------+ |
- | | | |
- xxCxxxxxxxxxxCxxxxxCxxxCxxxxxxxxxCxxxxxxCxCxxxC
- *******************|***| | |
- +---|----------------+ |
- +------------------+
-
- 'C': conserved cysteine involved in a disulfide bond.
- '*': position of the pattern.
-
- -Consensus pattern: [KR]-x-C-x(3)-[SV]-x(2)-[FYWH]-x-[GF]-x-C-x(5)-C-x(3)-C
- [The four C's are involved in disulfide bonds]
- -Sequences known to belong to this class detected by the pattern: ALL.
- -Other sequence(s) detected in SWISS-PROT: NONE.
- -Last update: June 1994 / First entry.
-
- [ 1] Bruix M., Jimenez M.A., Santoro J., Gonzalez C., Colilla F.J., Mendez E.,
- Rico M.
- Biochemistry 32:715-724(1993).
- [ 2] Gu Q., Kawata E.E., Morse M.-J., Wu H.-M., Cheung A.Y.
- Mol. Gen. Genet. 234:89-96(1992).
- [ 3] Terras F.R.G., Torrekens S., van Leuven F., Osborn R.W., Vanderleyden J.,
- Cammue B.P.A., Broekaert W.F.
- FEBS Lett. 316:233-240(1993).
- [ 4] Bloch C. Jr., Richardson M.
- FEBS Lett. 279:101-104(1991).
- [ 5] Ishibashi N., Yamauchi D., Miniamikawa T.
- Plant Mol. Biol. 15:59-64(1990).
-