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- ************************
- * Clusterin signatures *
- ************************
-
- Clusterin is a vertebrate glycoprotein [1] which is known by various names:
-
- - In Human as complement-associated protein SP-40,40, complement cytolysis
- inhibitor (CLI), NA1/NA2, and apolipoprotein J (Apo-J).
- - In rat as sulfated glycoprotein 2 (SGP-2), dimeric acid glycoprotein (DAG),
- and Testosterone Repressed Prostate Message-2 (TRPM-2).
- - In bovine as glycoprotein III (GP-III).
- - In dog as glycoprotein 80 (gp-80).
- - In quail as protein T64.
-
- The exact function of this protein is not yet clear, it is known to be
- expressed in a variety of tissues and it seems to be able to bind to cells,
- membranes, and hydrophobic proteins. It has been associated with programmed
- cell death.
-
- Clusterin is synthesized as a precursor polypeptide of about 400 amino acids
- which is post-translationally cleaved to form two subunits (of about 200 amino
- acids each). The two subunits are linked by five disulfide bonds to form an
- antiparallel ladder-like structure [2]. In each of the mature subunits the
- five cysteines that are involved in disulfide bonds are clustered in domains
- of about 30 amino acids located in the central part of the subunits.
-
- We have developed two signature patterns for clusterin, each of them contains
- three cysteines, the first pattern is derived from the N-terminal subunit
- cluster and the second pattern is derived from the C-terminal subunit cluster.
-
- -Consensus pattern: C-K-P-C-L-K-x-T-C
- [The three C's are involved in disulfide bonds]
- -Sequences known to belong to this class detected by the pattern: ALL.
- -Other sequence(s) detected in SWISS-PROT: NONE.
-
- -Consensus pattern: C-L-[RK]-M-[RK]-x-[EQ]-C-[ED]-K-C
- [The three C's are involved in disulfide bonds]
- -Sequences known to belong to this class detected by the pattern: ALL.
- -Other sequence(s) detected in SWISS-PROT: NONE.
-
- -Expert(s) to contact by email: Peitsch M.C.
- mcp13936@ggr.co.uk
-
- -Last update: June 1994 / Text revised.
-
- [ 1] Jenne D.E., Tschopp J.
- Trends Biochem. Sci. 17:154-159(1992).
- [ 2] Choi-Miura N.H., Takahashi Y., Nakano Y., Tobe T., Tomita M.
- J. Biochem. 112:557-561(1992).
-