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- *****************************
- * Pectinesterase signatures *
- *****************************
-
- Pectinesterase (EC 3.1.1.11) (pectin methylesterase) catalyzes the hydrolysis
- of pectin into pectate and methanol. In plants, it plays an important role in
- cell wall metabolism during fruit ripening. In plant bacterial pathogens such
- as Erwinia carotovora and in fungal pathogens such as Aspergillus niger,
- pectinesterase is involved in maceration and soft-rotting of plant tissue.
-
- Prokaryotic and eukaryotic pectinesterases share a few regions of sequence
- similarity [1,2,3]. We selected two of these regions as signature patterns.
- The first is based on a region in the N-terminal section of these enzymes; it
- contains a conserved tyrosine which may play a role in the catalytic mechanism
- [3]. The second pattern corresponds to the best conserved region, an
- octapeptide located in the central part of these enzymes.
-
- -Consensus pattern: [GST]-x(5)-[LIVM]-x-[LIVM]-x(2)-G-x-Y-[DNK]-E-x-[LIVM]-x-
- [LIVM]
- -Sequences known to belong to this class detected by the pattern: ALL.
- -Other sequence(s) detected in SWISS-PROT: NONE.
-
- -Consensus pattern: G-[STAD]-[LIVMT]-D-F-I-F-G
- -Sequences known to belong to this class detected by the pattern: ALL.
- -Other sequence(s) detected in SWISS-PROT: NONE.
-
- -Last update: December 1992 / Patterns and text revised.
-
- [ 1] Ray J., Knapp J., Grierson D., Bird C., Schuch W.
- Eur. J. Biochem. 174:119-124(1988).
- [ 2] Plastow G.S.
- Mol. Microbiol. 2:247-254(1988).
- [ 3] Markovic O., Joernvall H.
- Protein Sci. 1:1288-1292(1992).
-