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- Newsgroups: bionet.molbio.methds-reagnts
- Path: sparky!uunet!utcsri!torn!csd.unb.ca!news.ucs.mun.ca!kean.ucs.mun.ca!jgill
- From: jgill@kean.ucs.mun.ca
- Subject: problems with mrna quantitation
- Message-ID: <1993Jan25.125413.1@kean.ucs.mun.ca>
- Lines: 24
- Sender: usenet@news.ucs.mun.ca (NNTP server account)
- Organization: Memorial University. St.John's Nfld, Canada
- Date: Mon, 25 Jan 1993 15:24:13 GMT
-
-
- Fellow netters:
-
- I have recently analysed my results during a quantitative rna analysis.
- During my analysis I used rRNA as a standard for the year to compare my
- summer hormone mrna levels. I am dealing with temperate fish so I was
- expecting the summer hormone rna's : rRNA levels to be high during periods of
- rapid growth and the opposite to be the case during the winter months.
-
- However, I found just the opposite to take place. That is to say that the
- ratios were high during the winter and low during the summer. This is sort
- of an indication to me that in, winter flounder, the fish which I am
- presently studying rRNA varies considerably over the year. Would anyone
- know of another mRNA which remains constant throughout the year which I
- would be able to use as a standard.
-
- Also, I was wondering if anyone would know if it would be a good idea to
- add a foreign rna to the G.T.C. extraction mixture to show quantitative rna
- extraction when using different probes.
-
- thanx Juan
-
- E-mail Jgill@kean.mun.ucs.ca
-
-