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- Path: sparky!uunet!mcsun!uknet!daresbury!news
- From: PARSONS_A@snd01.pcr.co.uk
- Newsgroups: bionet.software
- Subject: Synchronisation Problem in GCG with PIR : Solution & description
- Message-ID: <1993Jan7.105853.21680@gserv1.dl.ac.uk>
- Date: 7 Jan 93 10:58:17 GMT
- Sender: list-admin@daresbury.ac.uk
- Distribution: bionet
- Lines: 41
- Original-To: BIO-SOFT <BIO-SOFT@UK.AC.DARESBURY>
-
- Hi Netfolk,
-
- David Mathog (Caltech) mailed me to post to the net what the solution to my
- problem was, as had thanked all those who had helped me. Reinhard Doelz
- (Basel) and Liz Cowe (Oxford) both gave me the answer to fix the problem but Liz
- also provided an in depth analysis of the cause - with her agreement I am
- posting her analysis and am also alerting MIPS who provided the copy of PIR
- in question.
-
- Viva la (le?) Net!
-
- Tony p.
-
-
- ==============================================================================
- To: parsons_a@uk.co.pcr.snd01
- Message-ID: <009662A5.93B2E8A0.15248@vax.path.ox.ac.uk>
- Subject: GCG syncoronization problem
-
- Tony,
-
- I have also seen this problem with the PIR files that I get from MIPS
- - is this where yours come from too ? The problem seems to be that an extra
- character is added to the end of some sequence lines - this can't be seen (or
- may appear as an odd control character) and the PIR programs all cope with it
- fine. The GCG FASTA program decides the sequence and index don't match and so
- stop - if you fetch the sequence that is dying (find this by runing fasta with
- /monitor=1) then you will see an extra residue added at random to the end of
- the sequence - well I did. But some GCG programs can skip this character so my
- solution was to recreate the GCG index files - I first used Chopup so I could
- get short enough lines to check there were no funnies but actually thing this
- step isn't necessary and you can just use dataset and dbindex.
-
- I tried getting help from GCG and PIR but both suggested that the
- funny character was being included by MIPS when they make the GCG index so i
- should contact them. By this time I'd done all the reformating so didn't
- bother!
-
- Liz Cowe
- Oxford University Molecular Biology Data Centre
- liz@vax.path.ox.ac.uk
-