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- Newsgroups: bionet.molbio.methds-reagnts
- Subject: supercoiled DNA migration in agrose ?
- Message-ID: <1992Dec23.111137.24858@wehi.edu.au>
- From: chai_z@wehi.edu.au
- Date: 23 Dec 92 11:11:36 +1000
- Organization: Walter & Eliza Hall Institute
- Lines: 14
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-
- Hi,
-
- Several batches of M13 RF DNA of mine have been cut by EcoRI or SmaI,
- etc. and then electrophoresized in agrose gel. Uncut DNA (supercoiled) of some
- batches migrated faster than the cut one (linear), while the others slower.
- Most likely, some batches of my DNA were nicked (open circular). Could any one
- tell me the correct agrose pattern of supercoiled, open circular and linear DNA?
- or tell me where to refer to?
-
- Thanks in advance.
-
-
- Zhonglin Chai
-