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- Newsgroups: bionet.molbio.methds-reagnts
- Subject: Re: Removing RNA from a mixture of protein and RNA
- Message-ID: <1992Dec19.202704.25718@wisipc.weizmann.ac.il>
- From: lihouba@dapsas1.weizmann.ac.il (Christo Houbaviy)
- Date: Sat, 19 Dec 1992 20:27:04 GMT
- Sender: news@wisipc.weizmann.ac.il
- References: <9212171610.AA02023@miranda.med.pitt.edu>
- Distribution: bionet
- Organization: Weizmann Institute of Science, Computing Centre
- Lines: 29
-
- In article <9212171610.AA02023@miranda.med.pitt.edu> bsh@MED.PITT.EDU (Basavaraju Shankarappa) writes:
- >
- > Hi folks:
- >
- > Sometime back there was a question about removing RNA contamination
- >from a purified protein solution. Among the many suggestions that were
- >provided including precipitating proteins selectively some were neat.
- >
- > There is one more way. US biochemicals sells a large list of
- >immobilized enzymes and RNase A is one of those. I think this would be
- >a good idea if you can purify the ribonucleotides away from the protein
- >or if it does not matter.
-
- I think that this is a _great_ idea. Getting rid of the
- ribonucleotides should be no problem - one can use gel filtration or
- ultrafiltration (e.g. Centricon).
-
- >Raj Shankarappa
- >Univ of Pittsburgh
- >bsh@med.pitt.edu
-
- Regards,
-
- Christo
-
- --
- Christo Houbaviy [lihouba@dapsas.weizmann.ac.il]
- Department of Molecular Genetics and Virology
- The Weizmann Institute of Science, Rehovot, Israel
-