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- Xref: sparky sci.bio:2757 bionet.plants:189
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- From: ajt@puffin.doc.ic.ac.uk (Tony Travis)
- Newsgroups: sci.bio,bionet.plants
- Subject: Re: cutting a leaf
- Message-ID: <1992Jul27.171245.24941@doc.ic.ac.uk>
- Date: 27 Jul 92 17:12:45 GMT
- References: <ARA.92Jul25104105@camelot.ai.mit.edu>
- Sender: usenet@doc.ic.ac.uk
- Distribution: sci
- Organization: Department of Computing, Imperial College, University of London, UK.
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- ara@zurich.ai.mit.edu (Allan Adler) writes:
- : [...]
- : Alas I have no pith. I wouldn't know where to look for it. A more modern book
- : on histological technique assumes one will normally be using a microtome and
- : explains how to embed (human) tissues in paraffin and mount them
- : in the microtome. So I gather that the point is that I want something firm
- : enough to hold the object (leaf) I wish to cut and which is at the same time
- : soft enough that I can cut both it and the leaf, like a sandwich.
-
- The 'pith' is simply a region of undifferentiated cells inside the
- vascular cylinder of plant roots or stems.
-
- Many 'real' botanists cut a carrot in half and hold the leaf in that
- while they take gently tapering sections with a single-sided cut-throat
- razor.
-
- The thinnest part of the section (at the end of the taper) is thin
- enough to see the leaf anatomy clearly under a microscope. Mount the
- section in glycerine and use a cover slip to improve the visibility of
- the section.
-
- Tony
-
- --
- Dr. A.J.Travis, | Tony Travis
- Rowett Research Institute, | JANET: <ajt@uk.ac.sari.rri>
- Greenburn Road, Bucksburn, | other: <ajt@rri.sari.ac.uk>
- Aberdeen, AB2 9SB. UK. | phone: 0224-712751
-