Program options:

PCR product length, Minimum: Maximum:

Primer length, Minimum: Maximum:

Select forward primers only; reverse primers only.

Supress selection of PCR products.

Permit duplicate primer binding sites on template.

Primer DNA concentration: (nM)

Salt concentration: (mM)

Minimum % of specified PCR target range to be included

in PCR products: %

Primer G+C, Minimum: % Maximum: %

Primer melting temp., Minimum: C Maximum: C

Product G+C, Minimum: % Maximum: %

Product melting temp., Minimum: C Maximum: C

Other options: