SLIDE 26
(Nancy Pratt)

So, at this point we knew it was time for sperm. So here's the other end of the equipment. This is all JoGayle's little mobile sperm lab set up here by the giraffe enclosure, and she took this sperm out of the equitainer and warmed it up and assessed it under the microscope, and then we - she and Jennifer Buff, her technician, filled our syringes, and it was a go. And we inseminated Shanthi - I should tell you we did this twice. We did this two days apart, two different times with two different samples from Onyx. The first day we inseminated with 33 ml of semen - extended semen. It's yellow because of an extender, which has egg yolk in it, and that helps keep it alive and nourish it - it keeps it alive longer than it normally would stay alive. So we inseminated with 33 ml of this mixture to a total of about 8 billion live sperm, and on the second day about 43 mls, and it was a, I think I have that backwards - 43 mls was the first day, and that was about 8 billion sperm, and then 33 mls the second day, about 14 billion sperm. The second day was a bit more concentrated.