Document 0794
 DOCN  M9480794
 TI    Evaluation of the sensitivity, specificity, and predictive value of
       monoclonal antibody 3F6 for the detection of Pneumocystis carinii
       pneumonia in bronchoalveolar lavage specimens and induced sputum.
 DT    9410
 AU    Wazir JF; Macrorie SG; Coleman DV; Department of Cytopathology, Saint
       Mary's Hospital, London, UK.
 SO    Cytopathology. 1994 Apr;5(2):82-9. Unique Identifier : AIDSLINE
       MED/94312679
 AB    The sensitivity and specificity of different staining procedures for the
       detection of Pneumocystis carinii organisms were compared. Three
       conventional stains (Papanicolaou, Giemsa and Grocott's) and one
       immunocytochemical stain using 3F6 antibody were used on smears prepared
       from the same specimen. Bronchoalveolar lavage (BAL) and induced sputum
       (IS) specimens were used for this purpose. One hundred and sixty-five
       episodes from 142 patients were investigated by the four different
       staining techniques. Cysts of P. carinii were detected in 64 episodes
       from 63 patients. Immunocytochemical staining with 3F6 was found to be
       slightly more sensitive at detecting the cysts than Grocott's, Giemsa,
       or Papanicolaou stain.
 DE    Antibodies, Monoclonal/*DIAGNOSTIC USE  AIDS-Related Opportunistic
       Infections/DIAGNOSIS  Bronchoalveolar Lavage Fluid/*MICROBIOLOGY
       Evaluation Studies  Human  Immunohistochemistry  Pneumocystis
       carinii/ISOLATION & PURIF  Pneumonia, Pneumocystis carinii/*DIAGNOSIS
       Predictive Value of Tests  Sensitivity and Specificity
       Sputum/*MICROBIOLOGY  Stains and Staining  JOURNAL ARTICLE

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